Reporter

Part:BBa_K4242013:Design

Designed by: zongheng Wei   Group: iGEM22_CUG-China   (2022-09-29)


Pci+RBS+fleQ+TT+ Pci+Ppel+RBS+gfp+TT(pHYD-3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 943
    Illegal XhoI site found at 1396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 336
    Illegal NgoMIV site found at 1344
    Illegal NgoMIV site found at 1356
    Illegal NgoMIV site found at 1432
    Illegal NgoMIV site found at 1517
    Illegal AgeI site found at 745
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2567
    Illegal SapI.rc site found at 358
    Illegal SapI.rc site found at 1291
    Illegal SapI.rc site found at 1300


Design Notes

This is a composite part that uses gfp to detect the function of FleQ in the plasmid.


Source

Use RBS to produce FleQ protein under the Pci and use the GFP protein to reflect FleQ working under the control of Pci and Ppel in the plasmid.

References